With the Antibody Dynamics platform, investigators have access to technologies that allow the interrogation of the biophysical and functional properties of antibody isotypes and subclasses to a variety of global health diseases. These properties can lead to the identification of correlates of protection, which is essential for enabling product development and licensure of highly efficacious vaccines. Moreover, characterization of antibody responses can enable a greater understanding of the mechanistic underpinnings of immune protection, leading to further innovations in therapeutic and prevention strategies to improve human health.
The Antibody Dynamics platform uses customizable, antigen-specific binding, avidity, and functional assays in a GCLP-compliant environment to profile biophysical and functional antibody responses to infection and vaccination. Profiling is available for all antibody isotypes and subclasses and for several global health diseases, including malaria, TB, hepatitis B, tetanus, influenza virus, and HIV. These techniques can be used with small (µL) quantities of serum/plasma and mucosal samples from pre-clinical and clinical trials and can also be used for research and development with a variety of model organisms. We work closely with GHVAP Vaccine Statistical Support platform for optimal analytical power in deciphering correlates of protection.
GHVAP Antibody Dynamics offer:
Antibody Isotype and Subclass Profiling
- Profiling antibody isotypes (IgM, IgG, IgA)and subclasses (IgG1, IgG2, IgG3, IgG4, IgA1, IgA2) to multiple antigens simultaneously (up to 500 proteins/epitopes), including both linear and conformational epitopes.
Antibody Avidity Measurements
- Avidity measurements of on-rate (ka), off-rate (kd), and dissociation constants (K,d) to profile the strength of antigen-antibody interactions using biolayer interferometry (BLI) and surface plasmon resonance (SPR).
Antibody Effector Function Assays
- Customized antibody effector function assays (e.g. antibody dependent cell mediated phagocytosis, FcR array binding/avidity) to evaluate mechanisms of correlates of immunity.