The antibody isolation & characterization platform partner's technology rapidly, accurately and comprehensively identifies the functional antibodies and T cell receptors in an immune response. The platform partner generates the complete variable region sequences of the natively paired immunoglobulin chains of single B and T cells in a high-throughput manner, so that the true, native antibodies and TCRs present in a patient are identified and can be expressed and studied. Applications include (but are not limited to): - evaluation and comparison of vaccine-induced immune responses
- delineation of the time course of the immune response
- identification of antibodies conferring protection
- discovery of the antigen targets of immune responses
- identification of biomarkers for protection
Suitable input materials include: - Cryopreserved peripheral blood mononuclear cells (viability 80% or higher)
- Single B cells sorted into microtiter plate
- Fresh tissue
Available outputs include: - High fidelity paired heavy and light chain (or TCR) sequences, suitable for direct use in recombinant protein expression
- T cell subset assignments
- Sequence annotation (e.g. V(D)J assignments, somatic mutations, isotypes, CDR and framework region coordinates)
- Clonal family assignments
- Phyogenetic trees representing evolutionary relationships between antibodies or TCRs
- Algorithmic prioritization of antibodies for recombinant protein expression
- Recombinant antibody protein (e.g. for activity assays, antigen identification etc.)
- Custom data analysis results
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