The antibody isolation & characterization platform partner's technology rapidly, accurately and comprehensively identifies the functional antibodies and T cell receptors in an immune response. The platform partner generates the complete variable region sequences of the natively paired immunoglobulin chains of single B and T cells in a high-throughput manner, so that the true, native antibodies and TCRs present in a patient are identified and can be expressed and studied.

Applications include (but are not limited to):

• evaluation and comparison of vaccine-induced immune responses
• delineation of the time course of the immune response
• identification of antibodies conferring protection
• discovery of the antigen targets of immune responses
• identification of biomarkers for protection

Suitable input materials include:

• Cryopreserved peripheral blood mononuclear cells (viability 80% or higher)
• Single B cells sorted into microtiter plate
• Fresh tissue

Available outputs include:

• High fidelity paired heavy and light chain (or TCR) sequences, suitable for direct use in recombinant protein expression
• T cell subset assignments
• Sequence annotation (e.g. V(D)J assignments, somatic mutations, isotypes, CDR and framework region coordinates)
• Clonal family assignments
• Phyogenetic trees representing evolutionary relationships between antibodies or TCRs
• Algorithmic prioritization of antibodies for recombinant protein expression
• Recombinant antibody protein (e.g. for activity assays, antigen identification etc.)
• Custom data analysis results